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For your reference we have cataloged below a number of published articles about the features, benefits, and uses of Hemo-De. We invite you to read them and learn how Hemo-De can help your laboratory. |
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| Title | Use of Hemo-De to eliminate toxic agents used for concentration and trichrome staining of intestinal parasites |
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| Authors | W.E. Aldeen and D. Hale Associated Regional and University Pathologists, Inc., Salt Lake City, Utah 84108. |
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| Publication | Journal of Clinical Microbiology, Jul 1992, 1893-1895, Vol 30, No. 7 Copyright © 1992 by the American Society for Microbiology. All rights reserved. |
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| Abstract | In a blind comparison, 465 randomly collected clinical fecal specimens were examined. Hemo-De was found to be an excellent replacement for ethyl acetate in the concentration procedure and for carbol-xylene and xylene in the trichrome staining procedure. Elimination of toxic reagents, combined with its lower cost, makes Hemo-De the preferred choice in routine parasitology examinations. | |
| Download | Full Article pdf | |
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| Title | Gutta-Percha Softening: "Hemo-De" as a Xylene Substitute. | |
| Authors | Metzger, Zvi DMD; Marian-Kfir, Vered DMD; Tamse, Aviad DMD Journal of Endodontics. 26(7):385-388, July 2000. |
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| Publication | Copyright © 2005, American Association of Endodontists. All rights reserved. Published by Lippincott Williams & Wilkins. |
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| Abstract | The putative hazardous nature of chloroform and xylene implies that safer substitutes should be considered. Standard cylindrical gutta-percha samples were immersed in Hemo-De, a xylene substitute, for 60 s at 37[degrees]C. Weight loss of a sample, after drying, was used as a measure of its solubility. Master and accessory cone gutta-percha of three brands were compared. The highest solubility of all kinds of gutta-percha was in chloroform, which served as a positive control. The average solubility of all samples in xylene and Hemo-De was 61% and 52% of that in chloroform, respectively. DMS gutta-percha was more soluble than of Hygienic and DeTrey. Master cone gutta-percha of all brands was more soluble than that of their accessory cones. These results indicate that (a) large differences exist in the solubility of gutta-percha and (b) Hemo-De dissolved gutta-percha in a range similar to that of xylene and may be considered as a potential substitute for this organic solvent. | |
| Download | Full Article pdf | |
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| Title | Use of a Single Slide Trichrome-Stained Concentrate for the Detection of intestinal Parasites Stained Concentration Procedure for Ova and Parasites |
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| Authors | Devon C. Hale, MD, Karen Carroll, MD, James R Kucera, MS, and William E. Aldeen, MBA American Journal of Clinical Pathology, Vol. 106, No. 2, August 1996. |
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| Publication | Copyright © 1996, American Society of Clinical Pathologits. All rights reserved. Published by Lippincott-Raven Publishers. |
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| Abstract | The detection and identification of intestinal parasites was studied using a single slide of a trichrome-stained concentrate made from a cupircpolyvinal alcohol (PVA)-preserved specimen. When saline was used in place of formalin in the concentration procedure, it was possible to adequately stain the concentrate with a modified-trichrome stain. This was compared to a more traditional two-slide ova and parasite (O&P) procedure. Of the 1,000 specimens evaluated, a total of 261 parasites were detected using the single slide, modified trichrome-stained concentrate compared to 249 from the formalin/Hemo-De wet mount and 226 from the unconcentrated trichrome stain. The detection rate of the single slide method was comparable to the combined detection rate of the two-slide method. The saline/Hemo-De concentration procedure has the advantage of using nontoxic saline in place of formaldehyde. This new method is more cost-effective because it requires the processing and evaluation of only one slide. | |
| Download | Full Article pdf | |
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| Title | Biodegradable, effective substitute for xylene in the Ehrlich indole procedure | |
| Authors | JM Miller, MD Miller, PE Driscoll and P Miller Diagnostic Microbiology Section, Centers for Disease Control and Prevention, Atlanta, GA 30333. |
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| Publication | Journal of Clinical Microbiology, Aug 1994, 2028-2030, Vol 32, No. 8 Copyright © 1994 by the American Society for Microbiology. All rights reserved. |
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| Abstract | Three extracting reagents were compared for effectiveness in the Ehrlich indole procedure: xylene (the recommended reagent), Hemo-De (a terpene-based product containing d-limonene), and Micro-Clear (an isoparaffinic hydrocarbon). Thirty-three strains representing 12 species of indole-positive aerobes or facultative anaerobes and 50 strains representing 11 species of indole-positive strict anaerobes were tested using the three reagents. Xylene extraction allowed indole detection in all of the isolates tested. Micro-Clear allowed detection in all of the aerobic isolates and in 49 of 50 anaerobes. Hemo-De allowed indole detection in 32 of 33 aerobes and in 49 of 50 anaerobes. There was no significant difference in the results among the reagents. Because Micro-Clear is biodegradable, nonflammable, noncarcinogenic, and odorless, we feel that this product should be considered a safe and effective substitute for xylene in the Ehrlich indole procedure. | |
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